\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907<\/a>\u662f\u7528\u4e8e\u68c0\u6d4b\u6c34\u8d28\u7684\u4eea\u5668\u548c\u8bbe\u5907\uff0c\u5176\u5206\u7c7b\u548c\u539f\u7406\u53ef\u4ee5\u6839\u636e\u68c0\u6d4b\u5bf9\u8c61\u3001\u68c0\u6d4b\u76ee\u7684\u548c\u68c0\u6d4b\u65b9\u6cd5\u7b49\u56e0\u7d20\u8fdb\u884c\u5206\u7c7b\u3002\u5728\u73b0\u4ee3\u5de5\u4e1a\u4e2d\uff0c\u6c34\u8d28\u76d1\u6d4b<\/a>\u8bbe\u5907\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6c34\u5904\u7406\u3001\u6d77\u6c34\u6de1\u5316\u3001\u5de5\u4e1a\u5e9f\u6c34\u5904\u7406\u3001\u98df\u54c1\u5de5\u4e1a\u3001\u751f\u7269\u533b\u836f\u7b49\u9886\u57df\u3002\u4e0b\u9762\u5c06\u5bf9\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u7684\u5206\u7c7b\u548c\u539f\u7406\u53ca\u5176\u5728\u73b0\u4ee3\u5de5\u4e1a\u4e2d\u7684\u5e94\u7528\u7814\u7a76\u8fdb\u884c\u8be6\u7ec6\u4ecb\u7ecd\u3002<\/p>\n \u4e00\u3001\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u7684\u5206\u7c7b<\/p>\n 1. \u7269\u7406\u68c0\u6d4b\u6cd5<\/p>\n \u7269\u7406\u68c0\u6d4b\u6cd5\u662f\u6307\u901a\u8fc7\u7269\u7406\u53cd\u5e94\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u4e00\u4e9b\u6210\u5206\uff0c\u5982\u8fc7\u6ee4\u3001\u5206\u79bb\u3001\u5438\u9644\u7b49\u3002\u5e38\u7528\u7684\u7269\u7406\u68c0\u6d4b\u6cd5\u5305\u62ec\u591a\u76f8\u6d41\u68c0\u6d4b\u6cd5\u3001\u79bb\u5b50\u68c0\u6d4b\u6cd5\u3001\u6d6e\u6e38\u68c0\u6d4b\u6cd5\u7b49\u3002<\/p>\n 2. \u5316\u5b66\u68c0\u6d4b\u6cd5<\/p>\n \u5316\u5b66\u68c0\u6d4b\u6cd5\u662f\u6307\u901a\u8fc7\u5316\u5b66\u53cd\u5e94\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u4e00\u4e9b\u6210\u5206\uff0c\u5982\u6c27\u5316\u8fd8\u539f\u53cd\u5e94\u3001\u9178\u78b1\u5ea6\u3001PH\u503c\u7b49\u3002\u5e38\u7528\u7684\u5316\u5b66\u68c0\u6d4b\u6cd5\u5305\u62ec\u7535\u5316\u5b66\u68c0\u6d4b\u6cd5\u3001\u6c14\u76f8\u8272\u8c31\u6cd5\u3001\u6db2\u76f8\u8272\u8c31\u6cd5\u7b49\u3002<\/p>\n 3. \u751f\u7269\u68c0\u6d4b\u6cd5<\/p>\n \u751f\u7269\u68c0\u6d4b\u6cd5\u662f\u6307\u901a\u8fc7\u751f\u7269\u68c0\u6d4b\u6280\u672f\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u4e00\u4e9b\u6210\u5206\uff0c\u5982\u8367\u5149\u68c0\u6d4b\u6cd5\u3001PCR\u68c0\u6d4b\u6cd5\u7b49\u3002<\/p>\n \u4e8c\u3001\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u7684\u539f\u7406<\/p>\n 1. \u7269\u7406\u68c0\u6d4b\u6cd5<\/p>\n \u7269\u7406\u68c0\u6d4b\u6cd5\u4e3b\u8981\u662f\u901a\u8fc7\u7269\u7406\u53cd\u5e94\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u4e00\u4e9b\u6210\u5206\uff0c\u5982\u8fc7\u6ee4\u3001\u5206\u79bb\u3001\u5438\u9644\u7b49\u3002\u5e38\u89c1\u7684\u7269\u7406\u68c0\u6d4b\u65b9\u6cd5\u5305\u62ec\u591a\u76f8\u6d41\u68c0\u6d4b\u6cd5\u3001\u79bb\u5b50\u68c0\u6d4b\u6cd5\u3001\u6d6e\u6e38\u68c0\u6d4b\u6cd5\u7b49\u3002<\/p>\n \u591a\u76f8\u6d41\u68c0\u6d4b\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u591a\u6761\u7ba1\u9053\u7ec4\u6210\u7684\u7cfb\u7edf\uff0c\u5c06\u4e0d\u540c\u7684\u6c34\u6837\u6d41\u7ecf\u4e0d\u540c\u7684\u8fc7\u6ee4\u5668\u3001\u5206\u79bb\u5668\u7b49\u7ec4\u4ef6\uff0c\u4ee5\u68c0\u6d4b\u6c34\u8d28\u4e2d\u5404\u79cd\u6210\u5206\u7684\u5206\u5e03\u60c5\u51b5\u7684\u65b9\u6cd5\u3002\u79bb\u5b50\u68c0\u6d4b\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u79bb\u5b50\u6d53\u5ea6\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u5404\u79cd\u79bb\u5b50\u542b\u91cf\u7684\u65b9\u6cd5\u3002\u6d6e\u6e38\u68c0\u6d4b\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u68c0\u6d4b\u6c34\u4e2d\u6d6e\u6e38\u751f\u7269\u7684\u6570\u91cf\u548c\u79cd\u7c7b\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u751f\u7269\u542b\u91cf\u7684\u65b9\u6cd5\u3002<\/p>\n 2. \u5316\u5b66\u68c0\u6d4b\u6cd5<\/p>\n \u5316\u5b66\u68c0\u6d4b\u6cd5\u4e3b\u8981\u662f\u901a\u8fc7\u5316\u5b66\u53cd\u5e94\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u4e00\u4e9b\u6210\u5206\uff0c\u5982\u6c27\u5316\u8fd8\u539f\u53cd\u5e94\u3001\u9178\u78b1\u5ea6\u3001PH\u503c\u7b49\u3002\u5e38\u89c1\u7684\u5316\u5b66\u68c0\u6d4b\u65b9\u6cd5\u5305\u62ec\u7535\u5316\u5b66\u68c0\u6d4b\u6cd5\u3001\u6c14\u76f8\u8272\u8c31\u6cd5\u3001\u6db2\u76f8\u8272\u8c31\u6cd5\u7b49\u3002<\/p>\n \u7535\u5316\u5b66\u68c0\u6d4b\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u7535\u89e3\u6c34\u6837\uff0c\u68c0\u6d4b\u51fa\u5176\u4e2d\u7684\u67d0\u4e9b\u6210\u5206\uff0c\u5982\u6c22\u6c27\u6839\u79bb\u5b50\u3001\u6c22\u79bb\u5b50\u3001\u6c2f\u79bb\u5b50\u7b49\uff0c\u4ee5\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u5316\u5b66\u6c61\u67d3\u7684\u65b9\u6cd5\u3002\u6c14\u76f8\u8272\u8c31\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u6c14\u76f8\u8272\u8c31\u67f1\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u5404\u79cd\u6210\u5206\u7684\u65b9\u6cd5\u3002\u6db2\u76f8\u8272\u8c31\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u6db2\u76f8\u8272\u8c31\u67f1\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u5404\u79cd\u6210\u5206\u7684\u65b9\u6cd5\u3002<\/p>\n 3. \u751f\u7269\u68c0\u6d4b\u6cd5<\/p>\n \u751f\u7269\u68c0\u6d4b\u6cd5\u662f\u901a\u8fc7\u751f\u7269\u68c0\u6d4b\u6280\u672f\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u4e00\u4e9b\u6210\u5206\uff0c\u5982\u8367\u5149\u68c0\u6d4b\u6cd5\u3001PCR\u68c0\u6d4b\u6cd5\u7b49\u3002\u8367\u5149\u68c0\u6d4b\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7\u8367\u5149\u5242\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u67d0\u4e9b\u6210\u5206\u7684\u65b9\u6cd5\u3002PCR\u68c0\u6d4b\u6cd5\u662f\u4e00\u79cd\u901a\u8fc7PCR\u6280\u672f\u6765\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u67d0\u4e9b\u6210\u5206\u7684\u65b9\u6cd5\u3002<\/p>\n \u4e09\u3001\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u5728\u73b0\u4ee3\u5de5\u4e1a\u4e2d\u7684\u5e94\u7528\u7814\u7a76<\/p>\n 1. \u6c34\u5904\u7406<\/p>\n \u6c34\u5904\u7406\u662f\u5de5\u4e1a\u4e2d\u5fc5\u4e0d\u53ef\u5c11\u7684\u73af\u8282\uff0c\u800c\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u662f\u5b9e\u73b0\u6c34\u5904\u7406\u7684\u5173\u952e\u8bbe\u5907\u4e4b\u4e00\u3002\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u53ef\u4ee5\u68c0\u6d4b\u6c34\u4e2d\u7684\u786c\u5ea6\u3001PH\u503c\u3001\u6c28\u6c2e\u3001\u603b\u6c2e\u3001\u5316\u5b66\u9700\u6c27\u91cf\u7b49\u6210\u5206\uff0c\u4ee5\u68c0\u6d4b\u6c34\u4e2d\u7684\u6c61\u67d3\u7269\u8d28\u3002<\/p>\n 2. \u6d77\u6c34\u6de1\u5316<\/p>\n \u6d77\u6c34\u6de1\u5316\u662f\u5c06\u6d77\u6c34\u4e2d\u7684\u76d0\u5206\u964d\u4f4e\uff0c\u4ee5\u4fbf\u5f97\u5230\u6de1\u6c34\u7684\u8fc7\u7a0b\u3002\u6d77\u6c34\u6de1\u5316\u8fc7\u7a0b\u4e2d\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u7684\u4f7f\u7528\u975e\u5e38\u91cd\u8981\uff0c\u53ef\u4ee5\u6709\u6548\u68c0\u6d4b\u6d77\u6c34\u6de1\u5316\u8fc7\u7a0b\u4e2d\u4ea7\u751f\u7684\u5e9f\u6c34\u4e2d\u7684\u989c\u8272\u3001PH\u503c\u3001\u786c\u5ea6\u7b49\u6210\u5206\uff0c\u4ee5\u4fdd\u8bc1\u6d77\u6c34\u6de1\u5316\u7684\u8d28\u91cf\u548c\u6548\u679c\u3002<\/p>\n 3. \u5de5\u4e1a\u5e9f\u6c34\u5904\u7406<\/p>\n \u5de5\u4e1a\u5e9f\u6c34\u5904\u7406\u662f\u5de5\u4e1a\u4e2d\u666e\u904d\u5b58\u5728\u7684\u95ee\u9898\uff0c\u800c\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u53ef\u4ee5\u6709\u6548\u68c0\u6d4b\u5e9f\u6c34\u4e2d\u7684\u989c\u8272\u3001PH\u503c\u3001\u603b\u6c2e\u3001\u5316\u5b66\u9700\u6c27\u91cf\u7b49\u6210\u5206\uff0c\u4ee5\u786e\u5b9a\u5e9f\u6c34\u7684\u6c34\u8d28\u72b6\u51b5\uff0c\u5e76\u91c7\u53d6\u76f8\u5e94\u7684\u5904\u7406\u63aa\u65bd\u3002<\/p>\n 4. \u98df\u54c1\u5de5\u4e1a<\/p>\n \u98df\u54c1\u5de5\u4e1a\u662f\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u7684\u91cd\u8981\u5e94\u7528\u9886\u57df\u4e4b\u4e00\uff0c\u53ef\u4ee5\u68c0\u6d4b\u98df\u54c1\u4e2d\u7684\u91cd\u91d1\u5c5e\u3001\u7ec6\u83cc\u603b\u6570\u3001\u5927\u80a0\u6746\u83cc\u7b49\u6210\u5206\uff0c\u4ee5\u4fdd\u8bc1\u98df\u54c1\u7684\u8d28\u91cf\u548c\u5b89\u5168\u3002<\/p>\n 5. \u751f\u7269\u533b\u836f<\/p>\n \u751f\u7269\u533b\u836f\u9886\u57df\u4e5f\u662f\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u7684\u91cd\u8981\u5e94\u7528\u4e4b\u4e00\uff0c\u53ef\u4ee5\u68c0\u6d4b\u6c34\u4e2d\u7684\u7ec6\u83cc\u3001\u75c5\u6bd2\u3001\u86cb\u767d\u8d28\u7b49\u6210\u5206\uff0c\u4ee5\u786e\u5b9a\u6c34\u8d28\u7684\u5b89\u5168\u6027\u548c\u53ef\u9760\u6027\u3002<\/p>\n \u7efc\u4e0a\u6240\u8ff0\uff0c\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u662f\u7528\u4e8e\u68c0\u6d4b\u6c34\u8d28\u7684\u4eea\u5668\u548c\u8bbe\u5907\uff0c\u5176\u5206\u7c7b\u548c\u539f\u7406\u53ef\u4ee5\u6839\u636e\u68c0\u6d4b\u5bf9\u8c61\u3001\u68c0\u6d4b\u76ee\u7684\u548c\u68c0\u6d4b\u65b9\u6cd5\u7b49\u56e0\u7d20\u8fdb\u884c\u5206\u7c7b\u3002\u5728\u73b0\u4ee3\u5de5\u4e1a\u4e2d\uff0c\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6c34\u5904\u7406\u3001\u6d77\u6c34\u6de1\u5316\u3001\u5de5\u4e1a\u5e9f\u6c34\u5904\u7406\u3001\u98df\u54c1\u5de5\u4e1a\u3001\u751f\u7269\u533b\u836f\u7b49\u9886\u57df\u3002\u901a\u8fc7\u4f7f\u7528\u6c34\u8d28\u76d1\u6d4b\u8bbe\u5907\uff0c\u53ef\u4ee5\u6709\u6548\u68c0\u6d4b\u6c34\u8d28\u4e2d\u7684\u6c61\u67d3\u7269\u8d28\uff0c\u786e\u4fdd\u5de5\u4e1a\u3001\u519c\u4e1a\u3001\u533b\u7597\u7b49\u9886\u57df\u7528\u6c34\u7684\u5b89\u5168\u6027\u548c\u53ef\u9760\u6027\u3002<\/p>\n \u6587\u7ae0\u6765\u6e90\u4e8e\u7f51\u7edc\uff0c\u82e5\u6709\u4fb5\u6743\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec\u5220\u9664\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":" 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